کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8328500 1540205 2018 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Purification, molecular properties, structural characterization, and immunomodulatory activities of water soluble polysaccharides from Sargassum angustifolium
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Purification, molecular properties, structural characterization, and immunomodulatory activities of water soluble polysaccharides from Sargassum angustifolium
چکیده انگلیسی
Sulfated polysaccharides isolated from Sargassum angustifolium and purified to determine their structural characteristics and biological activities. Crude polysaccharides and fractions (F1 and F2) were chiefly composed of neutral sugars (49.4-68.5%) and sulfates (12.5-23.0%) along with small amounts of uronic acids (1.3-13.6%) and proteins (4.1-4.7%). Polysaccharides were mainly constructed of different levels of fucose (23.9-69.9%) and galactose (22.5-29.8%) sugars. Subfractions with molecular weights ranging from 157.2 to 790.8 × 103 g/mol were identified for isolated polysaccharides. Polysaccharides induced RAW264.7 macrophage cells to release noticeable amounts of nitric oxide and cytokines including IL-1β, TNF-α, IL-6, IL-10 and IL-12 through NF-κB and MAPKs signaling pathways. Sulfate esters of fraction F2 were necessary to its bioactivity and they were located on carbons 2, 4 and 6 of the major sugars. Fraction F2 was formed of (1 → 4)- and (1 → 3)-linked fucose residues branched at C-2 and C-4 as well as (1 → 6)-linked galactose residues branched at C-3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 109, 1 April 2018, Pages 793-802
نویسندگان
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