کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8328674 | 1540207 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thermostabilization of a thermophilic 1,4-α-glucan branching enzyme through C-terminal truncation
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Thermostabilization of a thermophilic 1,4-α-glucan branching enzyme through C-terminal truncation Thermostabilization of a thermophilic 1,4-α-glucan branching enzyme through C-terminal truncation](/preview/png/8328674.png)
چکیده انگلیسی
Thermophilic proteins are useful for the detailed investigation of thermostability because they function efficiently at high temperatures. Comparison of the amino acid sequences and three-dimensional structures of mesophilic and thermophilic 1,4-α-glucan branching enzymes (GBEs) shows that the amino acid sequence of the last 26 residues at the C-terminal end of the GBE from Geobacillus thermoglucosidans STB02 (GBEGt, GenBank accession no. KJ660983) are not conserved, and that their 3-dimensional structure is flexible. These residues appear to be modified based upon a balance between flexibility and rigidity that is related to thermostability. In this study, a truncated mutant of GBEGt made by removing the last 26 residues from its C-terminal end was found to have increased thermostability and solubility, compared with the wild-type enzyme. Additionally, truncation of a portion of the C-terminus resulted in a decrease in aqueous stability. The circular dichroism spectra of GBEGt and GBEGtÎC were also found to be different. These results suggest that deletion of flexible residues at the C-terminal end of GBEGt, which are located on the surface of the enzyme, enhances the thermostability of the enzyme without significantly compromising its enzymatic activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 107, Part B, February 2018, Pages 1510-1518
Journal: International Journal of Biological Macromolecules - Volume 107, Part B, February 2018, Pages 1510-1518
نویسندگان
Xiaofeng Ban, Yiting Liu, Yuzhu Zhang, Zhengbiao Gu, Caiming Li, Li Cheng, Yan Hong, Abhishek S. Dhoble, Zhaofeng Li,