Sulfated modification, characterization, and antioxidant and moisture absorption/retention activities of a soluble neutral polysaccharide from Enteromorpha prolifera

A purified polysaccharide from Enteromorpha prolifera (PEP) with a molecular mass of 147.8 kDa, was prepared by hot-water extraction method and purified by anion-exchange chromatography and gel filtration chromatography. Low Mw polysaccharide of E. prolifera (LEP, 44.8 kDa) was obtained by enzymatic degradation. Sulfated derivatives of the two different Mw polysaccharides were synthesized by chlorosulfonic acid/pyridine method, and the degrees of substitution of SPEP and SLEP were 0.57 and 0.81, respectively. Physicochemical properties and FT-IR spectra showed that enzymatic degradation and sulfated modification of polysaccharides were successful. 13C NMR analysis showed that the sulfate groups mainly attached to C-6 of rhamnose. Moreover, the study revealed that enzymatic degradation and sulfated modification of polysaccharides improved significantly superoxide, hydroxyl and DPPH radicals scavenging activities in vitro, and enhanced obviously moisture absorption/retention capacities. Therefore, these results demonstrated that molecular weight and sulfate groups had obvious effects on antioxidant activities and moisture absorption/retention abilities of the polysaccharides from E. prolifera.