کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8329686 1540228 2016 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Timesaving microwave assisted synthesis of insulin amyloid fibrils with enhanced nanofiber aspect ratio
ترجمه فارسی عنوان
سنتز فایبریل های آمیلوئید انسولین با افزایش نسبت نانو فایبر به کمک مایکروویو به کار می رود
کلمات کلیدی
فیبریل انسولین، اشعه مایکروویو، نسبت ابعاد، روش ذخیره سازی،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Insulin amyloid fibrils with enhanced aspect ratio, were prepared using a timesaving microwave assisted (MW) methodology, reducing the incubation time from 13 to 2 h. The fibrillation process was followed indirectly by Thioflavin T Fluorescence and UV-vis analysis, by measuring the amount of β-sheets formed and the insulin present in solution, respectively. TEM and AFM analysis revealed that the insulin fibrils obtained through the MW method, have very similar lengths but are much thinner than the ones obtained using the conventional method (CM). Additionally, it was verified that the nature of the peptides present in the final insulin fibrils was not affected by microwave irradiation. These morphological differences might reflect on noticeably enhanced mechanical and optical properties that can exploited on the development of advanced bionanomaterials.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 92, November 2016, Pages 225-231
نویسندگان
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