کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8329695 | 1540228 | 2016 | 59 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Biochemical and molecular characterization of new keratinoytic protease from Actinomadura viridilutea DZ50
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
A new extracellular thermostable keratinolytic protease, designated KERDZ, was purified and characterized from a thermophilic actinomycetes Actinomadura viridilutea DZ50 isolated from Algerian fishing port. The isolate exhibited high keratinase production when grown in chicken-feather meal media (18,000 U/ml) after 96-h of incubation at 45 °C. The enzyme was purified by ammonium sulfate precipitation (35⿿55%)-dialysis and heat treatment (30 min at 75 °C) followed by UNO S-1 FPLC cation exchange chromatography and size exclusion HPLC column. The biochemical characterizations carried on include physico-chemical determination and spectroscopic analysis. The MALDI-TOF/MS analysis revealed that the purified enzyme was a monomer with a molecular mass of 19536.10-Da. The sequence of the 25 N-terminal residues of KERDZ showed high homology with those of actinomycetes keratinases. Optimal activity was achieved at pH 11 and 80 °C. KERDZ was completely inhibited by PMSF and DFP suggested its belonging to the serine keratinase family. KERDZ displayed higher levels of hydrolysis and catalytic efficiency than bacterial keratinases (KERAK-29, Actinase E, and KERAB) and subtilisins (subtilisin Carlsberg and subtilisin Novo). The kerDZ gene encoding KERDZ was isolated and its DNA sequence was determined. These properties make KERDZ a potential, promising and eco-friendly alternative to the conventional chemicals used for industrial applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 92, November 2016, Pages 299-315
Journal: International Journal of Biological Macromolecules - Volume 92, November 2016, Pages 299-315
نویسندگان
Mouna Ben Elhoul, Nadia Zaraî Jaouadi, Hatem Rekik, Maroua Omrane Benmrad, Sondes Mechri, Emna Moujehed, Sidali Kourdali, Mohamed El Hattab, Abdelmalek Badis, Samir Bejar, Bassem Jaouadi,