کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8329704 | 1540228 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Efficacy of carnosine on activation of caspase 3 and human renal carcinoma cell inhibition
ترجمه فارسی عنوان
اثربخشی کارنوسین در فعال شدن مهار کاسپاز 3 و سلول کارسینومای کلیه انسانی
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Carnosine is a natural antioxidant dipeptide that is highly concentrated in muscles and brain. The present study investigated the effect of carnosine cell growth inhibition and activation of the caspase-3 enzyme under cell co-culture system. Renal carcinoma and normal cells were co-cultured to provide three-dimensional views for the experimental analyses. Carnosine inhibited renal cancer cell growth up to 40%, whereas it was 25% in normal cells. Caspase-3 enzyme activity corresponded to the appearance of immunofluorescence in the cytoplasm using the caspase-3 antibody. Caspase-3 enzyme activity gradually increased in renal carcinoma cells in a concentration-dependent manner. The increased immunofluorescence and fluorescent detection of caspase-3 indicated the occurrence of apoptosis. The binding affinity of carnosine with caspase-3 subunit was confirmed by In silico docking study and glide energy was â5.2Â kcal/mol. Taking all these data together, it is suggested that the carnosine may be a potential antiproliferative agent in renal carcinoma tumor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 92, November 2016, Pages 377-382
Journal: International Journal of Biological Macromolecules - Volume 92, November 2016, Pages 377-382
نویسندگان
Muthuraman Pandurangan, Bhupendra Mistry, Gansukh Enkhataivan, Doo Hwan Kim,