کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8330273 | 1540238 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In silico mining and characterization of bifidobacterial lipoprotein with CHAP domain secreted in an aggregated form
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In silico mining and characterization of bifidobacterial lipoprotein with CHAP domain secreted in an aggregated form In silico mining and characterization of bifidobacterial lipoprotein with CHAP domain secreted in an aggregated form](/preview/png/8330273.png)
چکیده انگلیسی
Bifidobacterium breve C50 secretes a lipoprotein associated with glucose, acting in an aggregating form (>600Â kDa) as an agonist of TLR2/6. Similar lipoproteins were sought for in bifidobacteria. In silico, the closest homology was shown with a Bifidobacterium longum protein containing CHAP and lipobox domains. Two strains secreted aggregates whose peptides sequences aligned with the mined protein. C16:0 and C18:0 fatty acids detected in the aggregates further supported a lipoprotein structure. Glucose and mannose detected by gas chromatography were likely ligands of the lipoprotein. The binding of aggregates to galectin-1 indicated that hexosamines and galactose surrounded them. However, unlike B. breve C50, aggregate secreted by B. longum CBi0703 was unable to bind TLR2/6 likely because of a more hydrophobic structure. In gnotobiotic mice, the intake of B. longum aggregate induced, in splenic dendritic cells, the expression of genes involved in antigen presentation. A positive correlation between the number of dendritic cells and CD4+CD25+ cells was observed in mice receiving these aggregates. In conclusion, B. longum secretes a lipoprotein forming aggregates which may influence dendritic and CD4+CD25+ cell interactions independently of the TLR2/6 pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 653-662
Journal: International Journal of Biological Macromolecules - Volume 82, January 2016, Pages 653-662
نویسندگان
Angelo Scuotto, Pierre-Charles Romond, Serge Djorie, Monique Alric, Marie-Bénédicte Romond,