کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8332269 | 1540246 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzyme-assisted extraction of anticoagulant polysaccharide from Liparis tessellatus eggs
ترجمه فارسی عنوان
استخراج آنزیمی پلی ساکارید ضد انعقادی از تخم مرغ لیپارس تسلولاتوس
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
This study aimed to recover a heparin-like anticoagulant polysaccharide from Liparis tessellatus eggs (PLE) by using enzyme-assisted extraction technique. Extraction experiments were carried out using three different enzymes (Alcalase®2.4L, Flavourzyme®500MG, and Protamex®) under different conditions of temperature (45, 50, and 55 °C), pH (6.5, 7.0, and 7.5), incubation time (24, 36, and 48 h), and enzyme to substrate ratio (E/S = 0.5, 1.0, and 1.5%, w/w), which were combined according to a D-optimal design. Statistical analysis of extraction results allowed identifying the variables with greater influence on the extraction yield, and selecting the conditions that maximize the PLE extraction. The best extraction results were achieved when using the Protamex® enzyme in an E/S ratio of 1.34% (w/w), pH 6.60, 47.40 °C, during 26.50 h. Under these conditions, a polysaccharide yield of 2.10% (w/w) was obtained. Clotting time measurements, activated partial thromboplastin time, and prothrombin time for evaluation of the anticoagulant properties of PLE were determined and showed increasing activities in correlation with the concentrations used. In the final step, the heparin-like nature of PLE was confirmed by digestion with heparinases I, II, and III, which showed ÎDiHS-0S, ÎDiHS-6S, ÎDiHS-diS1, and ÎDiHS-diS2 at compositions of 0.04, 0.03, 0.35, and 0.24 mol/g, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 74, March 2015, Pages 601-607
Journal: International Journal of Biological Macromolecules - Volume 74, March 2015, Pages 601-607
نویسندگان
Bernadeth F. Ticar, Zuliyati Rohmah, Carmelo V. Ambut, Yeung-Joon Choi, Solange I. Mussatto, Byeong-Dae Choi,