کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8333490 1540260 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anti-oxidant activity of holo- and apo-c-phycocyanin and their protective effects on human erythrocytes
ترجمه فارسی عنوان
فعالیت ضد اکسید کننده هلو و آپو فسکوسیانین و اثرات محافظتی آن بر اریتروسیت های انسانی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
This study was conducted to investigate the anti-oxidant activity of the recombinant apo-c-phycocyanin (c-PC) β-subunit compared to native c-PC purified from Spirulina sp. The gene encoding the β-subunit of c-PC was successfully cloned and expressed in Escherichia coli. The anti-oxidant capacities of recombinant apo-c-PC(β) and native c-PC were evaluated by measuring their Trolox equivalent antioxidant capacities and examining their protective effects on erythrocytes from normal and homozygous haemoglobin E individuals against peroxyl radicals and hydrogen peroxide. The results demonstrated that the anti-oxidant capacities are native c-PC ≫ Trolox > recombinant apo-c-PC(β). Both anti-oxidant proteins can potentially protect erythrocytes from oxidative damage. Expression of c-PC in bacteria reduces the cost and time for protein production, and the recombinant protein could be further developed to obtain a more efficient protein for therapeutic purposes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 60, September 2013, Pages 393-398
نویسندگان
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