کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8333766 | 1540263 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Myelin basic protein: Structural characterization of spherulites formation and preventive action of trehalose
ترجمه فارسی عنوان
پروتئین پایه میلین: ویژگی ساختاری شکل گیری اسفرولیت ها و جلوگیری از ترهالوز
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Myelin basic protein (MBP) is one of the main protein components of central nervous system that as shown in our work, under appropriate condition, forms spherulites. The structural and morphological features of these elements and the ability of trehalose to decrease or completely avoid their formation have been clarified with different but complementary techniques. The FTIR spectra provided compelling evidence for the presence of a small amount of elements organized in secondary structures such as helices and sheets. Polarized optical microscope observations show the presence of spherulites with an average size ranged from 41 to 61 μm, characterized by non-birifrangent core. The fluorescence data supply useful informations that are consistent with the presence of a molecular exclusion effect, following interaction of the disaccharide with protein that involves the environment around the single tryptophan residue. Moreover, study of protein conformational states by SDS-PAGE, let us to state that trehalose completely avoid autocatalytic cleavage properties of MBP up to 4 days of incubation at 37 °C and pH 7.4.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 57, June 2013, Pages 63-68
Journal: International Journal of Biological Macromolecules - Volume 57, June 2013, Pages 63-68
نویسندگان
Cinzia Di Salvo, Davide Barreca, Giuseppina Laganà , Marcella di Bella, Ester Tellone, Silvana Ficarra, Ersilia Bellocco,