کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8333852 1540262 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antioxidant and anti-inflammatory activities of Radix Isatidis polysaccharide in murine alveolar macrophages
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Antioxidant and anti-inflammatory activities of Radix Isatidis polysaccharide in murine alveolar macrophages
چکیده انگلیسی
Radix Isatidis is an official herbal medicine for treatment of infection and inflammation in China. In this study, a novel heteropolysaccharide (RIWP) was isolated from R. Isatidis through DEAE Sepharose Fast Flow column and Sepharose CL-6B column. RIWP had a molecular weight of 57 kDa and was mainly composed of glucose, galactose and arabinose with a relative molar ratio of 2.0:1.1:1.0. The cytoprotective, antioxidant and anti-inflammatory properties of RIWP in lipopolysaccharide (LPS) stimulated murine alveolar macrophages were first reported here. Pretreatment with RIWP was found to potently prolong cell survival and repress the generation of reactive oxygen species (ROS) and lipid peroxidation after LPS-stimulation in murine alveolar macrophages. Furthermore antioxidant status was significantly deteriorated in the LPS-treated alveolar macrophages, such as low superoxide dismutase (SOD) activity and G-SH content, which was effectively restored by RIWP supplementation. More importantly, RIWP significantly suppressed LPS-induced increase in nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) production in murine alveolar macrophages. Additionally RIWP recovered mitochondrial membrane potential to normal conditions. All above response to LPS stimulation behaved in a concentration-dependent manner. This study provided evidences that RIWP appears to have the potential to prevent inflammatory disease in lung.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 58, July 2013, Pages 329-335
نویسندگان
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