کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8333964 1540266 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure-function analysis of a new bacterial lipase: Effect of local structure reorganization on lipase activity
ترجمه فارسی عنوان
تجزیه و تحلیل ساختار-عملکرد یک لیپاز باکتری جدید: تأثیر سازماندهی مجدد ساختار محلی بر فعالیت لیپاز
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Like many other bacterial lipases Pseudomonas aeruginosa lipase has a very strong structure-function relationship. Herein, the effect of structural compactness of lipase has been investigated on its activity. P. aeruginosa HR59 was isolated from burn infection as a new and inexpensive source. Bacterial lipase was extracted from this strain and purified by ion-exchange chromatography. Two divalent metal ions (calcium and magnesium) and three polar organic solvents (methanol, ethanol and iso-propanol) were used to study the structure-function relationship. Results of this study revealed that these additives activated enzyme by reducing the helix content of enzyme. Moreover, thermo stability of the enzyme decreased in the presence of calcium and magnesium ions; whereas it increased upon interaction with polar organic solvents under heat-induced denaturation. Results of this investigation encourage utilization of these activity enhancers for various medical and industrial applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 54, March 2013, Pages 180-185
نویسندگان
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