کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8334083 | 1540266 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
L-amino acid biosensor based on L-amino acid oxidase immobilized onto NiHCNFe/c-MWCNT/PPy/GC electrode
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: L-amino acid biosensor based on L-amino acid oxidase immobilized onto NiHCNFe/c-MWCNT/PPy/GC electrode L-amino acid biosensor based on L-amino acid oxidase immobilized onto NiHCNFe/c-MWCNT/PPy/GC electrode](/preview/png/8334083.png)
چکیده انگلیسی
A method is described for construction of a highly sensitive amperometric L-amino acid biosensor based on covalent immobilization of goat kidney L-amino acid oxidase (LAAO) onto carboxylated multiwalled carbon nanotubes/nickel hexacyanoferrate/polypyrrole hybrid film electrodeposited on surface of glassy carbon electrode. The biosensor showed optimum response within 5 s at pH 7.5 and 35 °C, when polarized at 0.15 V vs. Ag/AgCl. There was a linear relationship between biosensor response (μA) and L-phenylalanine concentration in the range, 0.5 μM to 100 mM. The sensitivity of the biosensor was 79.31 nA cmâ2 μMâ1 with a detection limit of 0.5 μM (S/N = 3). The enzyme electrode was used 160 times over a period of 140 days, when stored dry at 4 °C. The biosensor was evaluated and employed for determination of L-amino acid level in fruit juices and alcoholic beverages. The biosensor showed excellent analytical characteristics along with short response time, good shelf life, and negligible interference of commonly present interferents. The film could be used for improvement of other biosensors also.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 54, March 2013, Pages 250-257
Journal: International Journal of Biological Macromolecules - Volume 54, March 2013, Pages 250-257
نویسندگان
Suman Lata, C.S. Pundir,