کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8334352 1540269 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural changes of glucose oxidase upon interaction with gold-coated magnetic nano-particles
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structural changes of glucose oxidase upon interaction with gold-coated magnetic nano-particles
چکیده انگلیسی
Structural changes of glucose oxidase (GOx), as a typical protein, upon interaction with gold-coated magnetic nano-particles (GMNPs) were studied. GMNPs were characterized by UV-vis, FTIR and SEM. Due to GMNPs interaction, obvious changes in enzyme conformation and its secondary and tertiary structures were verified using intrinsic fluorescence and circular dichroism. Moreover, Zeta potential measurement showed GMNPs surface charge shift to more negative value due to interaction with GOx. Finally, the electrochemical results revealed that while the enzyme electron transfer rate on GMNPs is higher, this process on cysteamin-GMNPs is more reversible.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 51, Issue 5, December 2012, Pages 998-1002
نویسندگان
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