Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense

► A purified polysaccharide fraction was obtained from Ganoderma sinense. ► Its backbone is mainly structured of 1-, 1,4-, 1,6-linked-β-d-glucopyranosyl. ► It showed significantly stimulating effect on the cytokine secretion from PBMC. ► It could also stimulate the NO and the cytokine secretion from raw 264.7 cell.