کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8334843 | 1540275 | 2012 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
In this study, a novel heteropolysaccharide was isolated from the fruiting bodies of Boletus speciosus Forst through DEAE-cellulose column and Sephadex G-200 column. The Boletus speciosus Forst polysaccharide (BSFP-1) had a molecular weight of 1.33 Ã 104 Da and was mainly composed of l-Man and d-Gal which ratios were 2:1. Structural features of Boletus speciosus Forst polysaccharide (BSFP-1) were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The results indicated that Boletus speciosus Forst polysaccharide (BSFP-1) had a backbone of (1â4)-α-l-mannopyranose residues which branches at O-6 based on the experimental results. The branches were mainly composed of one with â1)-α-d-galactopyranose residue. The antioxidant activity of BSFP-1 was evaluated with two biochemical methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPHâ) radical scavenging, scavenging activity of 2,2â²-azino-bis(3-ethylbenzthiazoline-6-suphonic acid)diammonium (ABTS+) radical. The results indicated that BSFP-1 showed strong antioxidant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 3, 1 April 2012, Pages 613-618
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 3, 1 April 2012, Pages 613-618
نویسندگان
Xiang Ding, Yi-ling Hou, Wan-ru Hou,