کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8334843 1540275 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Structure elucidation and antioxidant activity of a novel polysaccharide isolated from Boletus speciosus Forst
چکیده انگلیسی
In this study, a novel heteropolysaccharide was isolated from the fruiting bodies of Boletus speciosus Forst through DEAE-cellulose column and Sephadex G-200 column. The Boletus speciosus Forst polysaccharide (BSFP-1) had a molecular weight of 1.33 × 104 Da and was mainly composed of l-Man and d-Gal which ratios were 2:1. Structural features of Boletus speciosus Forst polysaccharide (BSFP-1) were investigated by a combination of total hydrolysis, methylation analysis, gas chromatography-mass spectrometry (GC-MS), infrared (IR) spectra and nuclear magnetic resonance (NMR) spectroscopy. The results indicated that Boletus speciosus Forst polysaccharide (BSFP-1) had a backbone of (1→4)-α-l-mannopyranose residues which branches at O-6 based on the experimental results. The branches were mainly composed of one with →1)-α-d-galactopyranose residue. The antioxidant activity of BSFP-1 was evaluated with two biochemical methods, including 1,1-diphenyl-2-picrylhydrazyl (DPPH−) radical scavenging, scavenging activity of 2,2′-azino-bis(3-ethylbenzthiazoline-6-suphonic acid)diammonium (ABTS+) radical. The results indicated that BSFP-1 showed strong antioxidant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 3, 1 April 2012, Pages 613-618
نویسندگان
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