کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8335314 1540277 2012 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization and application of a detergent-stable alkaline α-amylase from Bacillus subtilis strain AS-S01a
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Characterization and application of a detergent-stable alkaline α-amylase from Bacillus subtilis strain AS-S01a
چکیده انگلیسی
A strain AS-S01a, capable of producing high-titer alkaline α-amylase, was isolated from a soil sample of Assam, India and was taxonomically identified as Bacillus subtilis strain AS-S01a. Optimized α-amylase yield by response surface method (RSM) was obtained as 799.0 U with a specific activity of 201.0 U/mg in a process control bioreactor. A 21.0 kDa alkaline α-amylase purified from this strain showed optimum activity at 55 °C and pH 9.0, and it produced high molecular weight oligosaccharides including small amount of glucose from starch as the end product. The Km and Vmax values for this enzyme towards starch were determined as 1.9 mg/ml and 198.21 μmol/min/mg, respectively. The purified α-amylase retained its activity in presence of oxidant, surfactants, EDTA and various commercial laundry detergents, thus advocating its suitability for various industrial applications.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 50, Issue 1, 1 January 2012, Pages 219-229
نویسندگان
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