کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336556 | 1540635 | 2016 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quercetin metabolites inhibit MMP-2 expression in A549 lung cancer cells by PPAR-γ associated mechanisms
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کلمات کلیدی
PPAR-γMMP-2TIMP-2Nm23-H1Q3GTGZQuercetin-3-glucuronideTroglitazone - ترولیدازونLung cancer cells - سلول های سرطانی ریهmatrix metalloproteinases-2 - ماتریکس فلزوپروتئیناز-2Metastasis - متاستاز Tissue inhibitor of metalloproteinase 2 - مهار کننده بافت متالوپروتئیناز 2Peroxisome proliferator-activated receptor gamma - گاما گیرنده گیرنده فعال پرولیفیزوم فعال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Our previous study demonstrated that quercetin-metabolite-enriched plasma (QP) but not quercetin itself upregulates peroxisome proliferator-activated receptor gamma (PPAR-γ) expression to induce G2/M arrest in A549 cells. In the present study, we incubated A549 cells with QP as well as quercetin-3-glucuronide (Q3G) and quercetin-3â²-sulfate (Q3â²S), two major metabolites of quercetin, to investigate the effects of quercetin metabolites on cell invasion and migration, the possible mechanisms and the role of PPAR-γ. We also compared the effects of QP with those of quercetin and troglitazone (TGZ), a PPAR-γ ligand. The results showed that QP significantly suppressed cell invasion and migration, as well as matrix metalloproteinases (MMPs)-2 activity and expression in a dose-dependent manner. The effects of 10% QP on those parameters were similar to those of 10 μM quercetin and 20 μM TGZ. However, QP and TGZ rather than quercetin itself increased the expressions of nm23-H1 and tissue inhibitor of metalloproteinase (TIMP-2). Furthermore, we demonstrated that Q3G and Q3â²S also inhibited the protein expression of MMP-2. GW9662, a PPAR-γ antagonist, significantly diminished such an effect of Q3G and Q3â²S. Silencing PPAR-γ expression in A549 cells also significantly diminished the suppression effect of Q3G and Q3â²S on MMP-2 expression. Taken together, our study demonstrated that QP inhibited cell invasion and migration through nm23-H1/TIMP-2/MMP-2 associated mechanisms. The upregulation of PPAR-γ by quercetin metabolites such as Q3G and Q3â²S could play an important role in the effects of QP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 33, July 2016, Pages 45-53
Journal: The Journal of Nutritional Biochemistry - Volume 33, July 2016, Pages 45-53
نویسندگان
Cheng-Hung Chuang, Chiao-Lin Yeh, Shu-Lan Yeh, En-Shyh Lin, Li-Yu Wang, Ying-Hsuna Wang,