کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336803 | 1540642 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ï-6 (18:2) and Ï-3 (18:3) fatty acids in reconstituted high-density lipoproteins show different functionality of anti-atherosclerotic properties and embryo toxicity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Among unsaturated fatty acids, epidemiologic studies have demonstrated that Ï-6 (linoleic acid) and Ï-3 (linolenic acid) fatty acids show different associations with risk of cardiovascular disease (CVD), although its molecular mechanisms remain unclear. To determine why consumption of Ï-3 fatty acid is associated with lower risk of CVD, we investigated the biological functions of Ï-6 (linoleic acid) and Ï-3 (linolenic acid) in reconstituted HDL (rHDL) after encapsulation using human cells and zebrafish embryo. Apolipoprotein A-I (apoA-I) is the principal protein behind the beneficial functions of HDL, which include potent anti-oxidant, anti-inflammatory, and anti-atherosclerotic activities in blood. Several rHDLs were synthesized with apoA-I and different molar ratio of Ï-6 or Ï-3 fatty acid. Both fatty acids showed similar solubility in rHDL up to a molar ratio of 95:5:1:10 (palmitoyloleoyl phosphatidylcholine:cholesterol:apoA-I:fatty acid). Although both rHDL showed similar structural properties and α-helical contents, Ï-6-rHDL showed loss of anti-oxidant ability against LDL oxidation. Uptake of acetylated LDL into macrophages was inhibited by Ï-3-rHDL but not Ï-6-rHDL, suggesting that Ï-6-rHDL has higher pro-atherosclerotic activity. Ï-3-rHDL showed more enhanced cholesterol efflux activity with less accumulation of triglyceride in the macrophage. Ï-6-rHDL caused more senescence in human dermal fibroblast cells with cytotoxicity, while Ï-3-rHDL treatment inhibited the senescence. In zebrafish embryo survivability, Ï-3-rHDL-injected embryos showed 86±3% survival, whereas Ï-6-rHDL-injected ones showed 72±2% survival as well as an elevated inflammatory response in zebrafish embryos. In conclusion, Ï-6-rHDL and Ï-3-rHDL show different physiological activities in atherosclerosis, inflammation, and cellular senescence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 12, December 2015, Pages 1613-1621
Journal: The Journal of Nutritional Biochemistry - Volume 26, Issue 12, December 2015, Pages 1613-1621
نویسندگان
Ki-Hoon Park, Jae-Yong Kim, Inho Choi, Jae-Ryong Kim, Kyung-Hyun Cho,