کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8338030 | 1540986 | 2016 | 17 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An improved micro-method for the measurement of steroid profiles by APPI-LC-MS/MS and its use in assessing diurnal effects on steroid concentrations and optimizing the diagnosis and treatment of adrenal insufficiency and CAH
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کلمات کلیدی
PCOS11-DOCDHEAACTH11-Deoxycortisol21-deoxycortisolCAHDHTTCCdehydroepiandrosteroneLOQHSDAPPI17α-Hydroxyprogesterone - 17α-هیدروکسی پروژسترون3α-hydroxysteroid dehydrogenase - 3α-هیدروکسیستروئید دهیدروژنازLC–MS/MS - LC-MS / MSAtmospheric pressure photoionization - photoionization فشار فشار اتمسفریinternal standard - استاندارد داخلیSteroids - استروئیدDiurnal variation - تنوع روزانهLOD یا Limit of detection - حد تشخیصDihydrotestosterone - دی هیدروتستوسترونpolycystic ovarian syndrome - سندرم تخمدان پلی کیستیکlower limit of detection - محدودیت پایین تشخیصlower limit of quantitation - محدودیت پایین کمیتAdrenal insufficiency - نارسایی قلبی عروقیadrenocorticotropic hormone - هورمون adrenocorticotropicliquid chromatography–tandem mass spectrometry - کروماتوگرافی مایع و اسپکترومتری توده دو طرفه21-hydroxylase deficiency - کمبود 21 هیدروکسیلاز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Our goals were to (1) develop an improved micro-method usable for neonates for steroid profile measurements and a method to measure androsterone, a key steroid in the recently described androgen backdoor pathway together, with dehydroepiandrosterone and (2) to assess if dehydroepiandrosterone diurnal concentration fluctuations exist potentially necessitating strict adherence to time of blood sample draw and requirement of separate time-dependent reference intervals. Liquid chromatography-tandem mass spectrometry was performed with an atmospheric pressure photoionization source [1]. For each sample 50 μL (100 μL for the backdoor pathway) of serum was deproteinized by adding 75 μL (150 μL for the backdoor pathway) of acetonitrile containing the internal standards. After centrifugation, 75 μL (150 μL for the backdoor pathway) of supernatant was diluted with 250 μL of water and injected onto a Poroshell 120 EC-C8 column (SB-C8 column for the backdoor pathway). Within-run coefficients of variation ranged from 2.4 to 10.4% and between-day coefficients of variation from 2.9 to 11.2%. Comparison studies yielded correlation coefficient between 0.97 and 1.00 with recoveries of 90% or greater. Our methods analyze a 9 steroid profile and an additional 2 steroid profile (backdoor pathway) with minimal sample volume (usable in neonates optimizing early diagnosis of endocrinopathies and genetic diseases). Low limits of quantitation make these methods ideal for steroid measurement in women and prepubertal children. As diurnal variations of dehydroepiandrosterone and other steroids [2] concentrations are clinically significant we recommend that separate reference intervals be developed for 8 am, 8 pm, and midnight sample draws. The use of this approach in improving the diagnosis of patients with adrenal insufficiency and congenital adrenal hyperplasia is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 162, September 2016, Pages 110-116
Journal: The Journal of Steroid Biochemistry and Molecular Biology - Volume 162, September 2016, Pages 110-116
نویسندگان
Brian R. Stolze, Verena Gounden, Jianghong Gu, Elizabeth A. Elliott, Likhona S. Masika, Brent S. Abel, Deborah P. Merke, Monica C. Skarulis, Steven J. Soldin,