کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340061 1541190 2018 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Velocity landscape correlation resolves multiple flowing protein populations from fluorescence image time series
ترجمه فارسی عنوان
همبستگی چشم انداز سرعت، تعداد چندین پروتئین را از مجموعه های زمانی تصویر فلوئورسانس حل می کند
کلمات کلیدی
طیف سنجی همبستگی تصویر، چشم انداز سرعت، فلورسانس، تجزیه و تحلیل نوسانات، نقشه های برداری،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Molecular, vesicular and organellar flows are of fundamental importance for the delivery of nutrients and essential components used in cellular functions such as motility and division. With recent advances in fluorescence/super-resolution microscopy modalities we can resolve the movements of these objects at higher spatio-temporal resolutions and with better sensitivity. Previously, spatio-temporal image correlation spectroscopy has been applied to map molecular flows by correlation analysis of fluorescence fluctuations in image series. However, an underlying assumption of this approach is that the sampled time windows contain one dominant flowing component. Although this was true for most of the cases analyzed earlier, in some situations two or more different flowing populations can be present in the same spatio-temporal window. We introduce an approach, termed velocity landscape correlation (VLC), which detects and extracts multiple flow components present in a sampled image region via an extension of the correlation analysis of fluorescence intensity fluctuations. First we demonstrate theoretically how this approach works, test the performance of the method with a range of computer simulated image series with varying flow dynamics. Finally we apply VLC to study variable fluxing of STIM1 proteins on microtubules connected to the plasma membrane of Cystic Fibrosis Bronchial Epithelial (CFBE) cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volumes 140–141, 1 May 2018, Pages 126-139
نویسندگان
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