کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340229 1541221 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Tuning the Music: Acoustic Force Spectroscopy (AFS) 2.0
چکیده انگلیسی
AFS is a recently introduced high-throughput single-molecule technique that allows studying structural and mechanochemical properties of many biomolecules in parallel. To further improve the method, we developed a modelling tool to optimize the layer thicknesses, and a calibration method to experimentally validate the modelled force profiles. After optimization, we are able to apply 350 pN on 4.5 μm polystyrene beads, without the use of an amplifier, at the coverslip side of the AFS chip. Furthermore, we present the use of a transparent piezo to generate the acoustic force and we show that AFS can be combined with high-NA oil or water-immersion objectives. With this set of developments AFS will be applicable to a broad range of single-molecule experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 105, 1 August 2016, Pages 26-33
نویسندگان
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