کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340347 1541228 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The origin of in situ hybridization - A personal history
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The origin of in situ hybridization - A personal history
چکیده انگلیسی
In situ hybridization is the technique by which specific RNA or DNA molecules are detected in cytological preparations. Basically it involves formation of a hybrid molecule between an endogenous single-stranded RNA or DNA in the cell and a complementary single-stranded RNA or DNA probe. In its original form the probe was labeled with 3H and the hybrid was detected by autoradiography. The first successful experiments in 1968 involved detection of the highly amplified ribosomal DNA in oocytes of the frog Xenopus, followed soon after by the reiterated “satellite DNA” in mouse and Drosophila chromosomes. Fluorescent probes were developed about ten years later.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 98, 1 April 2016, Pages 4-9
نویسندگان
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