کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340514 1541238 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanometric molecular separation measurements by single molecule photobleaching
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nanometric molecular separation measurements by single molecule photobleaching
چکیده انگلیسی
Although considerable progress has been made in imaging distances in cells below the diffraction limit using FRET and super-resolution microscopy, methods for determining the separation of macromolecules in the 10-50 nm range have been elusive. We have developed fluorophore localisation imaging with photobleaching (FLImP), based on the quantised bleaching of individual protein-bound dye molecules, to quantitate the molecular separations in oligomers and nanoscale clusters. We demonstrate the benefits of using our method in studying the nanometric organisation of the epidermal growth factor receptor in cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 88, 15 October 2015, Pages 76-80
نویسندگان
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