Physical characterization and profiling of airway epithelial derived exosomes using light scattering

Illustration of two light scattering measurements of HTBE exosomes (with mucin decoration on the surface) using DLS (left) and MALLS (right) techniques. As can be estimated from the picture the actual vesicular sphere is approximately 100 nm. The light scattering measurements, however, also include the mucin decoration in the estimate. Left: the hydrodynamic radius (Rh) of this particular vesicle will be equal to the radius of a sphere with the same diffusion coefficient as shown with the dotted line. Right: the radius of gyration (mean square root, MSR) is calculated using the mean distance of each radiated point (solid lines, R1-R6) from the center of the vesicle. DLS measures the Rh to be 340 nm, while MALLS measured the Rh to be 200 nm. The Rg/Rh is typically 0.6 for these vesicles.361