کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8340547 1541240 2015 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Next Generation Sequencing-based analysis of RNA polymerase functions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Next Generation Sequencing-based analysis of RNA polymerase functions
چکیده انگلیسی
Next Generation Sequencing (NGS) that revolutionized genome wide studies allows analysis of complex nucleic acids mixtures containing thousands of sequences. This extraordinary analytical power of NGS can be harnessed for the analysis of in vitro experiments where DNA template sequence dependence of protein activity acting on DNA can be studied in a single reaction for thousands of DNA sequence variants. This allows a rapid accumulation of data on DNA sequence dependence of the process of interest to a depth not accessible by standard experimentation. We use an example of bacterial RNA polymerase promoter melting activity to describe the NGS-based methodology to study DNA template dependence of protein activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 86, 15 September 2015, Pages 37-44
نویسندگان
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