Visualization of microbleeds with optical histology in mouse model of cerebral amyloid angiopathy

Optical histology enables co-registered, three-dimensional localization of the cerebral vasculature, cerebral amyloid angiopathy (CAA), and microbleeds, in a mouse model of CAA. (Top row, from left to right) 1) Tg2576 mouse brain section (~ 0.5 mm thick) after optical clearing, 2) cerebral blood vessels visualized with DiI fluorescence, 3) amyloid deposits visualized with Thioflavin S fluorescence. (Bottom row, from left to right) 4) microbleeds (located at tips of arrows) visualized with Prussian blue staining for hemosiderin in brightfield images, 5) microbleeds visualized with transmission microscopy, and 6) overlay of DiI, Thioflavin S and Prussian blue monochrome images.