Nitric oxide as an active substance in the enteric neurons of the porcine digestive tract in physiological conditions and under intoxication with bisphenol A (BPA)

Bisphenol A (BPA) is an organic substance, which is commonly used in the production of plastic. It is known that BPA has the negative impact on the living organism, affecting among others the reproductive organs, nervous, endocrine and immune systems. Nevertheless the knowledge about the influence of BPA on the enteric nervous system (ENS) is extremely scanty. On the other hand, nitric oxide is considered to be one of the most important neuronal factors in the ENS. The aim of the study was to investigate the influence of low and high doses of BPA on neuronal isoform nitric oxide synthase - like immunoreactive (nNOS-LI) nervous structures in the various parts of the porcine gastrointestinal (GI) tract using double immunofluorescence technique. The obtained results show that BPA affects nNOS-LI enteric neurons and nerve fibers, and the character and severity of observed changes depend on the fragment of the gastrointestinal tract, part of the ENS and dose of the toxin. It should be pointed out that even relatively low doses of BPA (0.05 mg/kg body weight/day) are not neutral for the organism and may change the number of nitrergic nervous structures in the stomach and intestine. Observed changes are probably connected with neurotoxic activity of BPA, but the exact mechanisms of them still remain unclear.