Proteomic analysis of the NOS2 interactome in human airway epithelial cells

► Proteomics identifies hundreds of NOS2-associated proteins in airway epithelial cells. ► Cytokines modulate the NOS2 interactome. ► Cytokines modulate NOS2 activity and ubiquitination. ► The ubiquitin ligase adapter FBXO45 is identified as a novel specific interactor with NOS2.