کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8347332 | 1541676 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection and quantitation of irisin in human cerebrospinal fluid by tandem mass spectrometry
ترجمه فارسی عنوان
تشخیص و اندازه گیری ایریسین در مایع مغزی نخاعی با استفاده از طیف سنجی توده ای
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کلمات کلیدی
NCENew England Biolabshigh-energy collision dissociationfibronectin type III domain containing 5FNDC5NEBPRMAQUAHCDACNBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز LC–MS/MS - LC-MS / MSAcetonitrile - استونیتریلnormalized collision energy - انرژی برخورد عادیThomson - تامسونCNS - دستگاه عصبی مرکزیblood brain barrier - سد خونی مغزیBBB - سد خونی مغزیcentral nervous system - سیستم عصبی مرکزیIrisin - شما ایریناMass spectrometry - طیف سنجی جرمیTandem mass spectrometry - طیف سنجی جرمی پشت سر هم یا متوالیLiquid chromatography-tandem mass spectrometry - طیف سنجی جرمی کروماتوگرافی مایع دو طرفهbrain derived neurotrophic factor - عامل مغز استخوان مغز استخوان استCerebrospinal fluid - مایع مغزی نخاعیCSF - مایع مغزی نخاعیParallel reaction monitoring - نظارت موازی موازیPlasma - پلاسماAbsolute quantification - کوانتومی مطلق
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The myokine irisin can cross the blood brain barrier and act as a neurokine to protect brain function during endurance exercise. However, the mechanism of transport from the blood to cerebrospinal fluid is unknown. Irisin has been detected in rodent and human brain and human cerebrospinal fluid by using commercial antibodies and enzyme linked immunosorbent assay kits. However, as human FNDC5 has an atypical translation start codon, some studies have questioned the specificity of commercial antibodies. Recently, human irisin was identified and quantitated in plasma by using mass spectrometry. We investigated whether there was irisin in human cerebrospinal fluid and an irisin concentration gradient between in human cerebrospinal fluid and paired plasma. An irisin peptide was identified and quantitated by using mass spectrometry with control peptides enriched with heavy stable isotopes as internal standards. Quantitative mass spectrometry identified the presence of irisin in human cerebrospinal fluid. The internal irisin peptides were modified to the deamidated asparagine form after deglycosylation. The unmodified internal irisin peptides were not found in CSF and irisin concentration was approximately 0.26-1.86â¯ng/ml in men over 80 years of age with various diseases. However, the parallel reaction monitoring (PRM) elution profiles of both modified and unmodified internal irisin peptides were not found in paired plasma samples. These data unequivocally demonstrated the presence of the glycosylated form of irisin in human cerebrospinal fluid. There were significant individual differences in men over 80 years of age with diseases. However, irisin was not detected in plasma samples by using mass spectrometry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 103, May 2018, Pages 60-64
Journal: Peptides - Volume 103, May 2018, Pages 60-64
نویسندگان
Qingwei Ruan, Limin Zhang, Jian Ruan, Xixue Zhang, Jie Chen, Cheng Ma, Zhuowei Yu,