کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8356478 | 1542018 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular and functional characterization of ShNAC1, an NAC transcription factor from Solanum habrochaites
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کلمات کلیدی
ACOSolanum habrochaitesQuantitative reverse-transcription PCRERFqRT-PCRNACACSORF1-aminocyclopropane-1-carboxylate oxidase - 1-آمینوسیلیکوپروپان-1-کربوکسیلات اکسیداز1-aminocyclopropane-1-carboxylate synthase - 1-آمینوسیلیکوپروپان-1-کربوکسیلات سنتازEthephon - اتفونEthylene - اتیلنeth - اخلاقAbiotic stress - استرس بی روحDEG - شماNAC transcription factor - فاکتور رونویسی NACopen reading frame - قاب خواندن بازMethyl viologen - متیل ویولونگwild-type - نوع وحشیGene ontology - هستیشناسی ژنیSenescence - پیریTomato - گوجه فرنگی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
NAC transcription factors (TFs) are important regulators of plant adaptation to abiotic stress. In this study, we functionally characterized an NAC TF, ShNAC1, from Solanum habrochaites. ShNAC1 was up-regulated by drought, cold, and salt stresses, and it displayed lower expression at the late stage of stress treatments than its orthologous gene in S. lycopersicum. Overexpression of ShNAC1 in tomato resulted in reduced cold, drought, and salt tolerance. Additionally, ShNAC1 displayed the highest expression in senescent leaf, and overexpressing ShNAC1 accelerated salt- and dark-induced leaf senescence. ShNAC1 was located in the nucleus without transactivation activity. RNA-seq analysis revealed that 81% (190 out of 234) differentially-expressed genes (DEGs) showed down-regulation in the transgenic line L2 compared with wild-type, suggesting that ShNAC1 may function as a transcriptional repressor. Among these down-regulated DEGs, many were involved in stress responses, such as SlHKT1;1, SlMAPKKK59, SlJA2, SlTIL, SlALDH2B1, etc. Noticeably, one ACS gene and three ACO genes involved in ethylene biosynthesis were up-regulated, while seven ERF genes in the ethylene signal transduction pathway were down-regulated in the transgenic lines, respectively. Our results suggested that ShNAC1 negatively regulates tolerance to abiotic stress in tomato probably by modulating the ethylene biosynthesis and signal transduction pathways.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 271, June 2018, Pages 9-19
Journal: Plant Science - Volume 271, June 2018, Pages 9-19
نویسندگان
Hui Liu, Yuhong Zhou, Hanxia Li, Taotao Wang, Junhong Zhang, Bo Ouyang, Zhibiao Ye,