Strigolactones and interaction with auxin regulating root elongation in tall fescue under different temperature regimes

Strigolactones (SL) have recently been found to play roles in regulating root development. However, it remains unclear how SL may mediate root elongation of perennial grass species under different temperatures that differentially affect root growth. The objectives of this study were to examine effects of SL on root elongation of tall fescue and to examine the interactive effects of SL and auxin in regulating root growth under both non-stress and heat stress conditions. Tall fescue (cv. 'Kentuck-31′) plants were treated with GR24 (a synthetic strigolactones), NAA (α-naphthylacetic acid), or NPA (auxin transport inhibitor N-1-naphthylphalamic acid) or their combination under non-stress control and heat stress (35/30 °C) in growth chamber. Crown root elongation was evaluated by measuring root length. Cell number and length in root tips were measured under confocal microscope. Expression levels of genes related to cell growth, SL signaling and auxin transport were determined. SL promoted crown root elongation in tall fescue under normal temperature and heat stress, and alleviated heat-inhibition of root growth. GR24-enhanced root elongation was accompanied with the increase in cell numbers, up-regulation of cell cycle-related genes, and down-regulation auxin transport-related genes in crown root tips of tall fescue. The positive effects of SL for promoting crown root elongation in tall fescue under both non-stress and heat stress could be mainly due to its regulation of cell division and involve the interference of auxin transport.