کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357307 | 1542047 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional characterization of GhSOC1 and GhMADS42 homologs from upland cotton (Gossypium hirsutum L.)
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کلمات کلیدی
DASFLCFULAP1SOC1SHORT VEGETATIVE PHASEGossypium hirsutumBiFCAPETALA1RT-PCRSUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1X-α-GalORFArabidopsis - آرابیدوپسیس یا رشادیchromatin immunoprecipitation - ایمن سازی کروماتینFlowering locus T - تلقیح توت Tbimolecular fluorescence complementation - تکمیل فلورسانس دو طرفه حرکتیgibberellic acid - جیبرلیک اسیدdays after sowing - روز بعد از کاشتlong day - روز طولانیRIN - رینopen reading frame - قاب خواندن بازSVP - لطفاyeast two-hybrid - مخمر دو رقمیreverse transcription-PCR - معکوس رونویسی PCRCHiP - چیپCol-0 - کل 0CONSTANS - کنستانسFLOWERING LOCUS C - گل LOCUS C
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In Arabidopsis flowering pathway, MADS-box genes encode transcription factors, with their structures and functions highly conserved in many species. In our study, two MADS-box genes GhSOC1 and GhMADS42 (Gossypium hirsutum L.) were cloned from upland cotton CCRI36 and transformed into Arabidopsis. GhSOC1 was additionally transformed into upland cotton. Comparative analysis demonstrated sequence conservation between GhSOC1 and GhMADS42 and genes of other plant species. Tissue-specific expression analysis of GhSOC1 and GhMADS42 revealed spatiotemporal expression patterns involving high transcript levels in leaves, shoot apical buds, and flowers. In addition, overexpression of both GhSOC1 and GhMADS42 in Arabidopsis accelerated flowering, with GhMADS42 transgenic plants showing abnormal floral organ phenotypes. Overexpression of GhSOC1 in upland cotton also produced variations in floral organs. Furthermore, chromatin immunoprecipitation assay demonstrated that GhSOC1 could regulate GhMADS41 and GhMADS42, but not FLOWERING LOCUS T, by directly binding to the genes promoter. Finally, yeast two-hybrid and bimolecular fluorescence complementation approaches were undertaken to better understand the interaction of GhSOC1 and other MADS-box factors. These experiments showed that GhSOC1 can interact with APETALA1/FRUITFULL-like proteins in cotton.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 242, January 2016, Pages 178-186
Journal: Plant Science - Volume 242, January 2016, Pages 178-186
نویسندگان
Xiaohong Zhang, Jianghui wei, Shuli Fan, Meizhen Song, Chaoyou Pang, Hengling Wei, Chengshe Wang, Shuxun Yu,