کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8357577 | 1542051 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Instability of the Arabidopsis mutant csn5a-2 caused by epigenetic modification of intronic T-DNA
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کلمات کلیدی
5-bromo-4-chloro-3-indolyl-phosphateGal4 activation domainNPT IIneomycin phosphotransferase II3-ATBCIPNBTBCART-qPCR3-amino-1,2,4-triazole - 3-آمینو-1،2،4-تریاازولAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییEpigenetic - اپی ژنتیکbicinchoninic acid - بیسینکنینیک اسیدNitro-blue tetrazolium - تترازولیم نیترو-آبیdouble mutant - جهش دوگانهDAG - روزdays after germination - روز بعد از جوانه زدنyeast two-hybrid - مخمر دو رقمیreverse transcriptase quantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی واکنش معکوسoptical density - چگالی نوری
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
T-DNA insertion mutants play a crucial role in elucidating Arabidopsis gene function. In some cases, two or more T-DNA mutants are combined to study genetic interactions between homologous genes or genes hypothesized to act in the same pathway. We studied the significance of protein-protein interactions between CSN5A and ROP11 by crossing three independent rop11 T-DNA insertion mutants with csn5a-2, a partial loss-of-function intronic T-DNA insertion mutant. The csn5a-2 single mutant is severely stunted, but double rop11 csn5a-2mutants were rescued and exhibited increased CSN5A transcript and protein levels. The rescued phenotype was maintained in non-Mendelian fashion when the csn5a-2 single mutant was re-isolated from the rop11-1 csn5a-2 double mutant, and was sensitive to two inhibitors of DNA methylation. Loss of kanamycin resistance was also observed in re-isolated csn5a-2. These findings indicate that the rescue of csn5a-2 resulted from a trans T-DNA-mediated epigenetic effect on the csn5a-2 intronic T-DNA, similar to recent reports involving the intronic T-DNA mutants ag-TD, ben1-1, and cob-6. Thus the work reported here provides further support for the recommendation that mutants created through novel combinations of T-DNA alleles should be carefully evaluated for evidence of epigenetic modification of T-DNA before final conclusions are drawn.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Science - Volume 238, September 2015, Pages 53-63
Journal: Plant Science - Volume 238, September 2015, Pages 53-63
نویسندگان
Xiaoyan Jia, Bidisha Chanda, Mingzhe Zhao, Amy M. Brunner, Eric P. Beers,