Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins: A case study of a bacterially expressed recombinant biopharmaceutical protein

Bubble plots showing the pI (x-axis), MW (y-axis) and amounts (bubble size) of HCPs determined by label-free absolute quantitation. The BP is represented by the purple dot. The amount of BP does not correspond to the size of the bubble.202