کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8359495 1542295 2018 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Kinetic properties analysis of beta-mannanase from Klebsiella oxytoca KUB-CW2-3 expressed in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Kinetic properties analysis of beta-mannanase from Klebsiella oxytoca KUB-CW2-3 expressed in Escherichia coli
چکیده انگلیسی
Endo-1,4-β-mannanase is an enzyme that can catalyze the random hydrolysis of β-1,4-mannosidic linkages in the main chain of mannans, glucomannans and galactomannans and offers many applications in different biotechnology industries. Purification and kinetic properties of the endo-1,4-β-mannanase from recombinant Escherichia coli strain KMAN-3 were examined. Recombinant β-mannanase (KMAN-3) was purified 50.5 fold using Ni-NTA Agarose resin and specific activity of 11900 U/mg protein was obtained. Purified KMAN-3 showed a single band on SDS-PAGE with a molecular weight of 43 kDa. Km and Vmax values of KMAN-3 on ivory nut mannan, locust bean gum, defatted copra meal and konjac glucomannan were 243, 3.83 × 105 37 and 2.13 × 106 mg ml−1 and 2940, 61,100, 3930 and 1.56 × 1010 mg−1, respectively. Carboxymethyl cellulose was not digested by KMAN-3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 146, June 2018, Pages 23-26
نویسندگان
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