کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8359607 1542298 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Production of the recombinant antimicrobial peptide UBI18-35 in Escherichia coli
چکیده انگلیسی
Radiolabeled peptides derived from ubiquicidine (UBI) are of great interest for early and highly accurate scintigraphic detection and differentiation of infection and sterile inflammation. In the present work the recombinant antimicrobial peptide UBI18-35 - a fragment of the human natural cationic peptide ubiquicidine - was produced in Escherichia coli for the first time. The insoluble expression of the peptide in fusion with ketosteroid isomerase provided high yield, about 6 mg of UBI18-35 per liter. We developed an approach to produce the antimicrobial peptide UBI18-35, that encompasses inclusion body isolation and size exclusion chromatography. This method could be the basis for industrial biotechnological production of diagnostic system components that are in high demand.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 143, March 2018, Pages 38-44
نویسندگان
, , , , , ,