کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8360094 1542327 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Optimizing conditions for production of high levels of soluble recombinant human growth hormone using Taguchi method
ترجمه فارسی عنوان
بهینه سازی شرایط تولید سطح بالایی از هورمون رشد نوترکیب انسانی محلول با استفاده از روش تاگوچی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی
Human growth hormone (hGH) is synthesized and stored by somatotroph cells of the anterior pituitary gland and can effect on body metabolism. This protein can be used to treat hGH deficiency, Prader-Willi syndrome and Turner syndrome. The limitations in current technology for soluble recombinant protein production, such as inclusion body formation, decrease its usage for therapeutic purposes. To achieve high levels of soluble form of recombinant human growth hormone (rhGH) we used suitable host strain, appropriate induction temperature, induction time and culture media composition. For this purpose, 32 experiments were designed using Taguchi method and the levels of produced proteins in all 32 experiments were evaluated primarily by ELISA and dot blotting and finally the purified rhGH protein products assessed by SDS-PAGE and Western blotting techniques. Our results indicate that media, bacterial strains, temperature and induction time have significant effects on the production of rhGH. The low cultivation temperature of 25 °C, TB media (with 3% ethanol and 0.6 M glycerol), Origami strain and a 10-h induction time increased the solubility of human growth hormone.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 114, October 2015, Pages 128-135
نویسندگان
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