کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8360279 | 1542333 | 2015 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins Application of coupled affinity-sizing chromatography for the detection of proteolyzed HSA-tagged proteins](/preview/png/8360279.png)
چکیده انگلیسی
Coupled affinity liquid chromatography and size exclusion chromatography (ALC-SEC) is a technique that has been shown to successfully report product quality of proteins during cell expression and prior to the commencement of downstream processing chromatography steps. This method was applied to monitoring the degradation and subsequent partial remediation of a HSA-tagged protein which showed proteolysis, allowing for rapid cell line development to address this product quality dilemma. This paper outlines the novel application of this method for measuring and addressing protease-induced proteolysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 108, April 2015, Pages 80-84
Journal: Protein Expression and Purification - Volume 108, April 2015, Pages 80-84
نویسندگان
Anne Serdakowski London, Kunal Patel, Lisa Quinn, Martin Lemmerer,