Expression, purification, and solubility optimization of peptidyl-tRNA hydrolase 1 from Bacillus cereus

- Peptidyl-tRNA hydrolase 1 from a novel pathogenic bacterial species Bacillus cereus.
- Recombinant E. coli expression of soluble protein optimized in M9 minimal media.
- Purified enzyme was well folded and active.
- Conditions for long term enzyme storage were determined.