Evaluation of four microbial Class II fructose 1,6-bisphosphate aldolase enzymes for use as biocatalysts

► Cloning and purification of four microbial Class II fructose 1,6-bisphosphate aldolases. ► Structural, stability, and functional characterization of aldolases as possible biocatalysts. ► Microbial aldolases were not more stable to temperature and organic solvents than the rabbit muscle aldolase.