Effects of orexin-monoaminergic interactions on oxytocin secretion in rat neurohypophyseal cell cultures

► The effects of orexin-monoaminerg interactions on oxytocin (OT) release were examined. ► We used rat neurohypophyseal cell cultures in our study. ►The OT level increased in response to monoaminergs or K+ treatment. ► Orexins reduced the adrenaline-, histamine- or serotonin-induced OT level increases. ► Orexin-monoaminerg interactions occur at both neurohypophyseal and hypothalamic levels.