کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8399174 1544419 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel computer vision algorithm for the reliable analysis of organelle morphology in whole cell 3D images - A pilot study for the quantitative evaluation of mitochondrial fragmentation in amyotrophic lateral sclerosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوفیزیک
پیش نمایش صفحه اول مقاله
Novel computer vision algorithm for the reliable analysis of organelle morphology in whole cell 3D images - A pilot study for the quantitative evaluation of mitochondrial fragmentation in amyotrophic lateral sclerosis
چکیده انگلیسی
Applying the algorithm we performed a pilot study in cultured motor neurons from transgenic G93A hSOD1 mice, a model of human familial amyotrophic lateral sclerosis. In the presence of the mutated SOD1 and upon excitotoxic treatment with kainate we demonstrate a clear fragmentation of the mitochondrial network, with an increase in the number of mitochondrial segments and a reduction in the length of mitochondria. Histogram analyses show a reduced number of tubular mitochondria and an increased number of small mitochondrial segments. The computer vision algorithm for the evaluation of organelle morphology allows an objective assessment of disease-related organelle phenotypes with greatly reduced examiner bias and will aid the evaluation of novel therapeutic strategies on a cellular level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mitochondrion - Volume 25, November 2015, Pages 49-59
نویسندگان
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