کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8403980 | 1544687 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Changes in external osmolality and ionic composition affect Megaleporinus obtusidens sperm motility
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Understanding the effects of environmental factors on sperm motility characteristics can increase artificial reproduction efficiency in species that do not spawn naturally in captivity, such as Megaleporinus obtusidens. This study evaluated the effects of the osmolality (25, 85, 145, 205, 265, and 325â¯mOsmâ¯kgâ1) and composition of activating solutions (NaCl, KCl, or fructose) on the percentage of motile sperm, and the swimming velocity and path straightness of M. obtusidens spermatozoa. The concentrations of major ions in the seminal fluid were also assessed and Na+ (74.46â¯mmolâ¯Lâ1), K+ (37.24â¯mmolâ¯Lâ1), and Clâ (114.29â¯mmolâ¯Lâ1) were the most abundant. When the activating solution was hypertonic (325â¯mOsmâ¯kgâ1) compared to the seminal fluid (293â¯mOsmâ¯kgâ1), sperm motility was completely inhibited. A wide range of osmolalities that initiated sperm motility were identified for all three solutions. Both, the percentage of motile sperm and the motility duration were reduced (Pâ¯<â¯.05) at extreme osmolalities. At 145â¯mOsmâ¯kgâ1, the percentage of motile sperm remained high (>50%) up to 40â¯s after activation and the motile phase lasted for >50â¯s, regardless of the activating solution composition. Over the postactivation time, the curvilinear velocity and straightness were similar (Pâ¯>â¯.05) for fructose and NaCl solutions, whereas KCl solutions induced a higher (Pâ¯<â¯.05) curvilinear velocity, lower (Pâ¯<â¯.05) straight-line velocity, and a circular swimming motion in spermatozoa. Our results suggest that a reduction in osmolality, using both non-electrolyte and electrolyte solutions, is the main trigger for the onset of spermatic movement in M. obtusidens sperm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 190, March 2018, Pages 63-74
Journal: Animal Reproduction Science - Volume 190, March 2018, Pages 63-74
نویسندگان
Jurandir Joaquim Bernardes Júnior, Jhon Edison Jimenez, Robie Allan Bombardelli, Alex Pires de Oliveira Nuñer,