کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405839 | 1544897 | 2018 | 24 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Decolorization of synthetic dyes by free and immobilized laccases from newly isolated strain Brevibacterium halotolerans N11 (KY883983)
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Extracellular laccase produced from a novel soil bacterium Brevibacterium halotolerans N11 (KY883983) was purified with molecular weight of 55â¯kDa. Different carriers such as Ca-alginate, agarose-agar, agar-agar, alginate-gelatin mixed gel and polyacrylamide gel were utilized for laccase immobilization through this study. Kinetic properties of immobilized laccase showed that, Km value was slightly increased while Vmax was decreased compared to free counterpart. The optimum pH and temperature values were increased from 5 and 35â¯Â°C to 6 and 40â¯Â°C of free and immobilized laccases, respectively. Also, the thermal stability of immobilized laccase is more than the free one with Tm values of 58â¯Â°C and 26â¯Â°C, respectively. The immobilized laccase could be reused for seven continuous cycles with retained 65% of its initial activity. Thus, the immobilization process improved the enzyme thermal stability, reusability and reduced the enzyme cost. Due to synthetic dyes released to the environments and their low biodegradability, this study focused on the potential role of free and immobilized laccases as agents for biodegradation of synthetic dyes, especially: azo, triarylmethane and anthraquinone dyes under different conditions. Therefore, the biodegradation of different synthetic dyes by free and immobilized laccases was also studied. Both the free and the immobilized laccases exhibited high decolorization efficiency to congo red dye (50â¯mgâ¯Lâ1) at 40â¯Â°C for free enzyme and 50â¯Â°C for immobilized laccase after 24â¯h and pH 5.5. Our results confirm the role of B. halotolerans N11 laccase in dye decolorization and wastewater detoxification.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 138-145
Journal: Biocatalysis and Agricultural Biotechnology - Volume 15, July 2018, Pages 138-145
نویسندگان
Fifi M. Reda, Noha S. Hassan, Abdel-Nasser El-Moghazy,