کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8405961 | 1544898 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization and application of fungal chlorogenate hydrolase to enzymatic breaking down of chlorogenate from yerba mate
ترجمه فارسی عنوان
تعیین و کاربرد کلروژناز قارچی هیدرولاز به انحلال آنزیم کلرژگانات از ایربا مات
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
The aim of this work was to study the physicochemical and kinetic properties of chlorogenate hydrolase (CHase) (EC 3.1.1.42) of Aspergillus niger AKU 3302 by induction in the presence of yerba mate extract. The data obtained could be applicable directly to mass processing for a plant scale production of quinic acid (QA) and caffeic acid (CA) with koji CHase. It is fundamentally significant to accumulate catalytic properties of CHase as much as possible with crude CHase, when koji CHase is directed to practical industrial processing of breakdown of chlorogenate (CGA). CHase exhibited a high thermal stability without appreciable loss after standing for 6â¯h at 50â¯Â°C and was stable in wide pH ranges from 2.0 to 9.0. The optimum pH and temperature of CHase activity were found at 6.5-7.0 and 45â¯Â°C, respectively. The Vmax and Km values for CGA hydrolysis were 1.996 unit mgâ1 and 0.72â¯mM, respectively. The activation energy of CHase was estimated to be 26â¯kJâ¯molâ1. Thus, CHase induced with CGA revealed successful for the production of CA and QA, fine chemicals of high interest and commercial values, from natural plant resources rich in CGA such as yerba mate. Several advantageous merits obtained with a crude CHase for the practical formation of QA and CA from yerba mate or yerba mate extract are discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 14, April 2018, Pages 395-401
Journal: Biocatalysis and Agricultural Biotechnology - Volume 14, April 2018, Pages 395-401
نویسندگان
Ana P. Butiuk, Silvana A. Maidana, MarÃa A. Martos, Yoshihiko Akakabe, Osao Adachi, Roque A. Hours,