کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8406115 | 1544899 | 2018 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Extraction, purification and characterization of phycoerythrin from Michrochaete and its biological activities
ترجمه فارسی عنوان
استخراج، تصفیه و خصوصی سازی فایکوئیرترین از میچرخاکت و فعالیت های بیولوژیکی آن
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کلمات کلیدی
فایکوئیرتین، پاکسازی، آنتی اکسیدان، ضد باکتری، ضد سرطان، فعالیت ضد قارچی،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Phycoerythrin, (PE) is red colored naturally abundant protein and is found in red algae and cyanobacteria. Till now, red algae are being characterized and exploited commercially for PE. But, cyanobacterial PE still need attention. PE is good for human health as it possess free radical scavenging ability and promotes human health. In present study PE was isolated and purified from cyanobacterium Michrochaete and was further tested for its antioxidant, antibacterial, antifungal and anticancer potential. Purified PE yielded two bands of 15.8 kDa and 17.7 kDa. Purity value of PE increased from 0.73 to 4.1 during successive purification steps. Purified PE showed anticancer activity against HepG2 cell line (IC50 = 105 µg/ml). Candida albicans was more resistant than Aspergillus niger at 0.2 mg/ml PE. Furthermore, PE showed antibacterial activity against both gram positive and gram negative bacterial species. The order of inhibitory activity was Pseudomonas aeruginosa (MTCC2543) > E.coli (ATCC 25922) > Staphylococcus aureus (MTCC902) with 0.1 mg/ml PE. Antioxidant activity of purified PE was evaluated by measuring the free radical scavenging ability using DPPH, ABTS and SOR. PE was found to have better free radical scavenging activity with ABTS (IC50 = 0.023 mg/ml) than DPPH (IC50 = 0.043 mg/ml) and SOR (IC50 = 0.553 mg/ml).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 13, January 2018, Pages 84-89
Journal: Biocatalysis and Agricultural Biotechnology - Volume 13, January 2018, Pages 84-89
نویسندگان
Hemlata Hemlata, Sumbul Afreen, Tasneem Fatma,