کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8416630 | 1545573 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A safe potential juice clarifying pectinase from Trichoderma viride EF-8 utilizing Egyptian onion skins
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A safe potential juice clarifying pectinase from Trichoderma viride EF-8 utilizing Egyptian onion skins A safe potential juice clarifying pectinase from Trichoderma viride EF-8 utilizing Egyptian onion skins](/preview/png/8416630.png)
چکیده انگلیسی
The production of a notable, safe and highly active pectinase by the local fungal strain Trichoderma viride EF-8 utilizing the abundant pigmented Egyptian onion (Allium cepa L.) skins (6.5%, w/v) was achieved in 4 days submerged fermentation (SMF) cultures, at temperature and pH of 30 °C and 4.0, respectively. The indigenously produced pectinase was partially purified by 50% batch ethanol precipitation and its general properties were studied following the standard procedures. The lyophilized enzyme preparation was free of any ochra or aflatoxins. The optimum conditions for the partially purified enzyme form were 2 mg/mL and 1% (w/v) enzyme protein and substrate (citrus pectin) concentrations, reaction pH and temperature of 7.0 and 40 °C, respectively. The results presented the low cost onion skins waste as the major substrate for the fungal pectinase production and its subsequent use in perfect fruit (apple, lemon and orange) juices clarification with remarkable stability during and after this process, which certainly enhance fruit juices processing in the tropics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 153-159
Journal: Journal of Genetic Engineering and Biotechnology - Volume 14, Issue 1, June 2016, Pages 153-159
نویسندگان
Abdel-Mohsen S. Ismail, Heba I. Abo-Elmagd, Manal M. Housseiny,