کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8418152 | 1545715 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunofiltration assay for aflatoxin B1 based on the separation of pre-immune complexes
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کلمات کلیدی
DABTween 20immunofiltration assay4CN3,3′-diaminobenzidine - 3،3'-diaminobenzidine4-chloro-1-naphthol - 4-کلرو-1-نافولAFB1 - AFB 1BSA - BSAAflatoxin B1 - آفلاتوکسین B1bovine serum albumin - آلبومین سرم گاوELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاSeparation - جداسازیpolyoxyethylenesorbitan monolaurate - پلی اتیلنزوربیتن مونولورات
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
A new approach for quantitative determination of AFB1 based on the separation of pre-immune complexes in the same immunoassay system has been developed. No additional step for the separation of pre-immune complexes is required. The method uses a test device for separation of pre-immune complexes from the free AFB1-enzyme conjugate by filtration through the membrane strips spotted with anti-AFB1 antibody. The bound enzyme conjugate was visualized by super-catalyzed reporter deposition (Super-CARD) signal amplification method. The measured signal intensity is directly proportional to the amount of AFB1 present in the sample. The detection limit obtained by the present method was 15Â pg/ml. The data on the analytical parameters indicate that the new format of AFB1 detection in foodstuffs is reproducible, accurate and specific. The method is user friendly and does not require any costly equipment or a well-equipped laboratory.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 392, Issues 1â2, 28 June 2013, Pages 24-28
Journal: Journal of Immunological Methods - Volume 392, Issues 1â2, 28 June 2013, Pages 24-28
نویسندگان
Debjani Saha, Dipika Roy, Tarun K. Dhar,