Isolation of microarray-quality RNA from primary human cells after intracellular immunostaining and fluorescence-activated cell sorting

► We describe a method to isolate microarray-quality RNA from cells purified by FACS. ► Cells were sorted after fixation, permeabilization and intracellular antibody staining ► Despite some degradation, recovered RNA performed remarkably well in microarray. ► It allows gene expression profiling of samples so far limited to single-gene study.